Part:BBa_K4825057:Design

pTDH3-ARO10-tTDH1-pPGK1-ADH7-tPGK1-pTEF2-ATF1-tSSA1

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2478
  Illegal EcoRI site found at 6138
  Illegal SpeI site found at 1429
  Illegal PstI site found at 1307
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2478
  Illegal EcoRI site found at 6138
  Illegal NheI site found at 4461
  Illegal SpeI site found at 1429
  Illegal PstI site found at 1307
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2478
  Illegal EcoRI site found at 6138
  Illegal BglII site found at 3967
  Illegal BglII site found at 5825
  Illegal BamHI site found at 4222
  Illegal BamHI site found at 7152
  Illegal XhoI site found at 2611
  Illegal XhoI site found at 7329
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2478
  Illegal EcoRI site found at 6138
  Illegal SpeI site found at 1429
  Illegal PstI site found at 1307
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2478
  Illegal EcoRI site found at 6138
  Illegal SpeI site found at 1429
  Illegal PstI site found at 1307
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2865
  Illegal BsaI site found at 7315
  Illegal BsaI.rc site found at 3977
  Illegal BsaI.rc site found at 7565
  Illegal SapI.rc site found at 6252
  Illegal SapI.rc site found at 7260
  

Design Notes

"HMG1, 3-hydroxy-3-methylglutaryl-CoA reductase, is the endogenous gene in yeast. It can catalyze the conversion from hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonate (MVA). By accumulating MVA through the over expression of tHMG1, MVA pathway is expressed more efficiently, and the yield of isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) would increase, which are both precursors of pinene, hence the yield of pinene can be increased. "

Source

Saccharomyces cerevisiae